Detection survey of mango seed weevil was undertaken throughout major mango production areas in six regions of Thailand. In each region, 1-2 provinces were selected as representatives of the region including province of Chiang Mai, Phichit, Nakorn Ratchasima, Ratchaburi, Petchaburi, Suphanburi, Chachoeng Sao and Chonburi. The survey was targeted on three mango cultivars of Namdork Mai, Thongdum and Chockanan which are the main cultivars exporting to Malaysia. Malaysian had agreed the methodology of survey protocol that was developed by Thailand. Fruit surveys were carried out in mango orchards during April to June 2009 to coincide with fruiting season. At least 600 fruits in each region were collected for mango seed weevil detection. All random sampling fruits were longitudinal cut open and dissection of seed for inspect the presence of mango weevils. Specimens found in infested fruits were collected and sent for identification by taxonomists at Insect Taxonomy Group, Entomology and Zoology Division, Plant Protection and Research Development Office, Department of Agriculture, Bangkok. Mango weevils collected from different areas were all identified to species and found to be Sternochetus olivieri (Faust). Therefore, result from the survey indicated that S. mangiferae is absence in Thailand.